Ruta Graveolens“DNA fragmentation and cell cycle arrest: a hallmark of apoptosis induced by Ruta graveolens in human colon cancer cells”.

Studio pubblicato a Gennaio 2015 sulla Rivista internazionale HOMEOPATHY – Published by Elsevier Ltd – da ricercatori della Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, India.

La ricerca ha indagato l’effetto anti-cancro di varie potenze di Ruta graveolens (Ruta) sulla linea cellulare COLO-205 (carcinoma del colon).

Nel trattamento delle cellule COLO-205 sono stati osservati effetti massimi con la tintura madre (MT) e con le potenze ultra diluite 30CH (ben oltre il numero di Avogadro), in cui sono state osservate diminuzioni della vitalità delle cellule cancerose, con ridotta capacità di clonogenesi e di migrazione.

Inoltre, nelle cellule COLO-205 trattate con Ruta Graveolens, sono state osservate alterazioni morfologiche e biochimiche a livello dei nuclei cellulari (frammentati con cromatina condensata) e aumentata quantità di DNA frammentato, che indicano la morte cellulare associata ad una attività apoptotica. La modalità di morte cellulare è stata suggestiva di un percorso apoptotico intrinseco e di arresto a G2 / M del ciclo cellulare.

Ruta Graveolens

Ruta Graveolens

Abstract
In the present study, we investigated the anti-cancer effect of various potencies of Ruta graveolens (Ruta) on COLO-205 cell line, as evidenced by cytotoxicity, migration, clonogenecity, morphological and biochemical changes and modification in the levels of genes associated with apoptosis and cell cycle. On treatment of COLO-205 cells maximal effects were seen with mother tincture (MT) and 30C potencies, wherein decrease in cell viability along with reduced clonogenecity and migration capabilities were noted. In addition morphological and biochemical alterations such as nuclear changes (fragmented nuclei with condensed chromatin) and DNA ladder-like pattern (increased amount of fragmented DNA) in COLO-205 cells indicating apoptotic related cell death were seen. The expression of apoptosis and cell-cycle related regulatory genes assessed by reverse transcriptase-PCR revealed an up-regulation of caspase 9, caspase-3, Bax, p21 and p27 expression and down-regulation of Bcl-2 expression in treated cells. The mode of cell death was suggestive of intrinsic apoptotic pathway along with cell cycle arrest at the G2/M of the cell cycle. Our findings indicate that phytochemicals present in Ruta showed potential for natural therapeutic product development for colon carcinoma.